BIO2133 LAB CHROMATOGRAM LAB REPORT

Topics: Drosophila melanogaster, Sunlight, Sex Pages: 3 (490 words) Published: January 13, 2015
Figure 1 compares the presence of various eye pigments found in both male and female Drosophila melanogaster by means of a 4-panelled bar graph. Eye pigments of five female and male mutant flies are compared to that of the wild type using Rf values (y-axis) from a chromatogram. The wild type (+) and only three of the five mutants are compared; brown (bw), scarlet (st), and rosy (ry). These four displayed pigments in both UV and visible light. No pigments were observed for white (w) and brown/scarlet (bw/st) eye mutations, and as a result were not included in the graph. Each panel displays Rf values correlating to the 7 different pigments observed; ommochrome, drosopterin, isoxanthopterin, xanthopterin, sepiapterin, 2-amino-4-hydroxypterine and biopterin. Panel A and B compare Rf values of the pigments of the four different male and female flies under UV light, whereas C and D make the same comparison for pigments observed under visible light. Panel A compares the eye pigments of wild type (+), brown (bw), scarlet (st) and rosy (ry) male Drosophila melanogaster observed under UV light by the comparison of the Rf values. These values are the distance the pigments travelled on the chromatogram. Paper chromatography allows us to separate the pigments, as each pigment has a different Rf value, thereby allowing us to identify the different pigments present in the wild and mutant type flies. For example, the presence of all pigments in the wild type(+), the absence of all pigments but ommochrome in the brown(bw) mutant, the presence of all pigments but ommochrome in the scarlet(st) mutant, and the presence of all pigments but isoxanthopterin is depicted in this panel. Panel B makes a similar comparison to panel A, except that this panel compares the eye pigments of the wild type(+), brown(bw), scarlet(st) and rosy(ry) female Drosophila melanogaster observed under UV light. In addition to the comparison of Rf values of the pigments in the wild type and mutant...
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